Part:BBa_B0032:Experience

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Characterizations of BBa_B0032

The Alverno_Ca team characterized BBa_K2066048, which includes BBa_B0032, as well as a series of other plasmids using TX-TL, a mean of in vitro transcription and translation using cell extract. Below is a graph of normalized expression of BBa_K2066048 in comparison to the other palsmids in the sequence.

This graph shows the fluorescence of the plasmids, over a period of 12 and a half hours, in a plate reader. "

Applications of BBa_B0032

William and Mary iGEM 2016

We characterized the strengths of the Anderson RBS library.

User Reviews

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••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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••••• UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5. See Experience page of BBa_B0034 for more details. Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter: RBS effpLux effpTet effJ23101 Declared efficiency B0030 0.40 1.6814 2.45 0,6 B0031 0.01 ND 0.04 0,07 B0032 0.19 0.4193 0.40 0,3 B0034 1 1 1 1 Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI: RBS effmRFP effAiiA effLuxI Declared efficiency B0030 1.72 0.96 0.45 0,6 B0031 0.03 0.78 0.028 0,07 B0032 0.37 0.51 N.D. 0.3 B0034 1 1 1 1

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